Analysis of 2-Aminobenzamide (AB) Labeled Glycans Using HPLC with Fluorescence Detection
ثبت نشده
چکیده
منابع مشابه
Rapid Preparation of Released N-Glycans for HILIC Analysis Using a Novel Fluorescence and MS-Active Labeling Reagent
Conventional approaches to the preparation of N-glycans for HILICFLR-MS are either laborious, time-consuming, or require compromises in sensitivity. In the case of one of the most frequently employed labeling compounds, 2-aminobenzamide (2-AB), the resulting glycans can be readily detected by fluorescence but are difficult to detect by electrospray ionization mass spectrometry (ESI-MS). Variati...
متن کاملUPLC/FLR/QTof MS Analysis of Procainamide-Labeled N-Glycans
UltraPerformance LC (UPLC) in hydrophilic interaction chromatography (HILIC) separation mode is becoming a routine and widely recognized technique for rapid, efficient, sensitive, and reproducible analysis of 2-aminobenzamide (2-AB) labeled glycans. Even though 2-AB is now the most common glycan labeling reagent, its use is limited by low MS sensitivity. Recent reports on LC/FLR/MS of glycans t...
متن کاملIdentification of carbohydrates binding to lectins by using surface plasmon resonance in combination with HPLC profiling.
A new, powerful method is presented for screening the binding in real time and taking place under dynamic conditions of oligosaccharides to lectins. The approach combines an SPR biosensor and HPLC profiling with fluorescence detection, and is applicable to complex mixtures of oligosaccharides in terms of ligand-fishing. Labeling the oligosaccharides with 2-aminobenzamide ensures a detection lev...
متن کاملComparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles—Part 1: Separation-based methods
Immunoglobulin G (IgG) crystallizable fragment (Fc) glycosylation is crucial for antibody effector functions, such as antibody-dependent cell-mediated cytotoxicity, and for their pharmacokinetic and pharmacodynamics behavior. To monitor the Fc-glycosylation in bioprocess development, as well as product characterization and release analytics, reliable techniques for glycosylation analysis are ne...
متن کاملN- and O-glycosylation analysis of etanercept using liquid chromatography and quadrupole time-of-flight mass spectrometry equipped with electron-transfer dissociation functionality.
Etanercept is a highly glycosylated therapeutic Fc-fusion protein that contains multiple N- and O-glycosylation sites. An in-depth characterization of the glycosylation of etanercept was carried out using liquid chromatography/mass spectrometry (LC/MS) methods in a systematic approach in which we analyzed the N- and O-linked glycans and located the occupied O-glycosylation sites. Etanercept was...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2011